Livers were analyzed from mice after BDL (n=6) or 12 times repeated CCl4 treatment (n=6) at indicated time points. (A, B) Hepatic mRNA levels of CCR2, MCP-1, MCP-2 and MCP-3 were measured after BDL (A) or CCl4 treatment (B) by qPCR. (C-E) CCR2 expression (in green) was detected by co-stained with desmin (in red, C), F4/80 (in red, D) and pan-Cytokeratin (in red, E), which was visualized by confocal microscopy. Arrows indicate CCR2-positive and desmin- (C) or F4/80-positive cells (D). The dashed lines indicate intrahepatic bile ducts (E). (F) CCR2 mRNA in Kupffer cells, HSCs and hepatocytes was measured by RT-PCR. β-actin mRNA served as an internal control.