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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Hepatology. 2009 Jul;50(1):185–197. doi: 10.1002/hep.22952

Figure 1. Expression of CCR2 and CCR2 ligands are increased in hepatic fibrosis.

Figure 1

Livers were analyzed from mice after BDL (n=6) or 12 times repeated CCl4 treatment (n=6) at indicated time points. (A, B) Hepatic mRNA levels of CCR2, MCP-1, MCP-2 and MCP-3 were measured after BDL (A) or CCl4 treatment (B) by qPCR. (C-E) CCR2 expression (in green) was detected by co-stained with desmin (in red, C), F4/80 (in red, D) and pan-Cytokeratin (in red, E), which was visualized by confocal microscopy. Arrows indicate CCR2-positive and desmin- (C) or F4/80-positive cells (D). The dashed lines indicate intrahepatic bile ducts (E). (F) CCR2 mRNA in Kupffer cells, HSCs and hepatocytes was measured by RT-PCR. β-actin mRNA served as an internal control.