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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Hepatology. 2009 Jul;50(1):185–197. doi: 10.1002/hep.22952

Figure 2. Reduction of hepatic fibrogenesis in CCR2-/- mice after BDL.

Figure 2

WT (n=6) and CCR2-/- (n=6) mice underwent BDL. (A) Fibrillar collagen deposition was evaluated by Sirius red staining and its quantification 21 days after BDL. (B) Hepatic hydroxyproline content was measured. (C, D) Expression of α-SMA was determined by immunohistochemistry (C) and western blotting (D). (E) Hepatic mRNA levels of collagen α1(I), α-SMA, TGFβ1 and TIMP-1 were measured in WT (n=5) and CCR2-/- (n=5) 5 days after BDL by qPCR. * p<0.05 for bile-duct ligated CCR2-/- mice versus bile-duct ligated WT mice. Original magnification is ×100 (A, C).