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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Hepatology. 2009 Jul;50(1):185–197. doi: 10.1002/hep.22952

Figure 5. CCR2 on bone marrow-derived cells is required for the early phase of macrophage infiltration after BDL.

Figure 5

Chimeric mice were generated by transplanting WT or CCR2-/- BM into irradiated and clodronate-treated WT mice or CCR2-/- mice (n=4-5 each group). Then chimeric mice were subjected to BDL for 5 days. (A) Successful BMT was confirmed by low levels of CCR2 mRNA in splenocytes from WT mice, but normal CCR2 mRNA in CCR2-/- mice transplanted with WT BM. (B) Hepatic mRNA levels of proinflammatory cytokines (TNF-α, MCP-1, RANTES and MIP-1β) in chimeric mice were measured by qPCR. (C, D) Macrophage infiltration was determined by immunohistochemistry for F4/80 (C) and qPCR for CD68 (D). (E) Serum ALT levels in chimeric mice 5 days after BDL were measured. Original magnification is ×200 (C). * p<0.05, ** p<0.01.