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. Author manuscript; available in PMC: 2010 Jul 1.

Published in final edited form as: Hepatology. 2009 Jul;50(1):185–197. doi: 10.1002/hep.22952

(A-F) Chimeric mice were generated by transplanting CCR2^-/- BM into irradiated and clodronate-treated WT mice (n=6) or CCR2^-/- mice (n=6) and vice versa (n=6 each group). 3 months after BMT, the mice were subjected to BDL and hepatic fibrosis was judged at 21 days after BDL. (A-B) Hepatic fibrosis in chimeric mice was assessed by Sirius red staining (A, left), quantitation (A, right), and hydroxyproline measurement (B). (C) α-SMA expression was shown by immunohistochemistry (C, upper) and western blotting (C, lower). (D) Macrophage infiltration was determined by immunohistochemistry for F4/80. (E) The accumulation of 4-hydoxy-nonenal was measured by immunohistochemistry. (F) Serum ALT levels in chimeric mice 21 days after BDL were measured. Original magnification is ×100 (A, C) and ×200 (D, E). * p<0.05, ** p<0.01.

(A-F) Chimeric mice were generated by transplanting CCR2^-/- BM into irradiated and clodronate-treated WT mice (n=6) or CCR2^-/- mice (n=6) and vice versa (n=6 each group). 3 months after BMT, the mice were subjected to BDL and hepatic fibrosis was judged at 21 days after BDL. (A-B) Hepatic fibrosis in chimeric mice was assessed by Sirius red staining (A, left), quantitation (A, right), and hydroxyproline measurement (B). (C) α-SMA expression was shown by immunohistochemistry (C, upper) and western blotting (C, lower). (D) Macrophage infiltration was determined by immunohistochemistry for F4/80. (E) The accumulation of 4-hydoxy-nonenal was measured by immunohistochemistry. (F) Serum ALT levels in chimeric mice 21 days after BDL were measured. Original magnification is ×100 (A, C) and ×200 (D, E). * p<0.05, ** p<0.01.