Figure 3.
rs4567(A) suppresses the translation of transfected ERManI in HeLa cells. (A) The translational efficiency of transfected ERManI (during a 20 minute pulse with 35S-methionine) in HeLa cells, with or without co-transfected Pi Z, were evaluated by a combination of immunoprecipitation, SDS-PAGE, autoradiography and quantified by PhosphoImager analysis. The error bars represent standard error of the mean. (B) The concentration of transfected ERManI (from above) under steady-state conditions was evaluated by Western blotting of cell lysates. The relative ERManI concentrations, as compared with control, were quantified in five separate experiments. The error bars represent a standard error of the mean.