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. Author manuscript; available in PMC: 2009 Jul 3.
Published in final edited form as: J Neurochem. 2007 Feb;100(3):780–789. doi: 10.1111/j.1471-4159.2006.04239.x

Fig. 5.

Fig. 5

Effect of treatment with 2 mM glutamate on wild-type (WT) hippocampal organotypic cultures in either the presence or the absence of 1 μM or 10 nM galanin, and/or 5 μM LY294009, and/or 300 nM PD98059. Cultures were then placed in serum-free media for a 24-h recovery period and imaged to measure the percentage of cell death in the CA1 region (solid bars) of the hippocampus. Results and representative images demonstrate an increase in cell death with either inhibitor after glutamate treatment. The protective effects of 1 μM galanin are abrogated with inhibitors of either the ERK or the Akt signalling pathways, whereas the protective effects of 10 nM galanin is abrogated by the Akt, but not the ERK, inhibitor. Bars represent mean ± SEM, n = 3. *Significantly different (p < 0.05) from glutamate-treated cultures; **significantly different (p < 0.01) from glutamate-treated cultures, +significantly different (p < 0.05) from galanin- and glutamate-treated cultures, ++significantly different (p < 0.01) from galanin- and glutamate-treated cultures.