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Morphologic, immunocytochemical, and cytofluorographic characteristics of cultured hMC. hMC harvested at 6–9 wk of culture were ≥95% metachromatic with toluidine blue (a) and were positive for tryptase (b) and chymase (c). Cytofluorographic assays (d) demonstrated strong c-kit expression, positive expression of CD13 and β3 integrin, and low expression of FcɛRI. Light tracings represent staining with negative controls; bold tracings represent staining for the designated markers.
