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. 2009 Jun 10;10(6):2662–2680. doi: 10.3390/ijms10062662

Figure 1.

Figure 1.

Panel A. Isothermal titration calorimetry data from radicicol binding to Hsp90αN. Upper graph – raw ITC data, lower graph – integrated ITC data with the curve fit to the standard single binding site model. The cell contained 4 μM protein, while the syringe contained 40 μM radicicol in the same buffer - 50 mM sodium phosphate, pH 7.5, 0.5% DMSO, 100 mM NaCl, at 25 °C. Panel B. Isothermal titration calorimetry displacement assay. All conditions are the same as in Panel A, except there was 1 mM compound 1 added to the calorimeter cell. Panel C. Chemical structures of radicicol and compound 1. Panel D. Isothermal titration calorimetry data from ethoxzolamide binding to hCAII. The cell contained 7 μM protein, while the syringe contained 100 μM ethoxzolamide in the same buffer - 50 mM sodium phosphate, pH 7.0, 0.5% DMSO, 50 mM NaCl, at 37 °C.