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. 2009 Jun 11;106(26):10746–10751. doi: 10.1073/pnas.0811817106

Fig. 4.

Fig. 4.

MiR-222 and miR-339 suppress gene expression by targeting the ICAM-1 3′-UTR. (A) U87 cells transfected with premiR for miR-222, miR-339, or both were cotransfected with psiCHECK-2 miR-222 WT (black columns on the Left), psiCHECK-2 miR-222 MT (white columns on the Left), psiCHECK-2 miR-339 WT (black columns on the Right), psiCHECK-2 miR-339 MT (white columns on the Right) or the control backbone psiCHECK-2 (gray columns on the Right). Renilla luciferase activity was normalized on the constitutive activity of firefly luciferase. The data represent mean ± SD of triplicate samples. **, P < 0.01 for U87 cells transfected with premiR-222/339 alone or ones cotransfected with both of premiR-222 and -339 compared with control premiR-transfected U87 cells. (B) ICAM-1 mRNA levels were assessed by quantitative RT-PCR in wild-type and U87 cells transfected with premiR-222, premiR-339, both premiRs, Dicer-siRNA, or appropriate negative control constructs. The relative ICAM-1 mRNA level for each sample was normalized to the β-actin mRNA level in each sample and calculated as the relative CT value to that of untreated U87 cells. The results represent mean ± SD of triplicate samples.