Fig. 5.
Electrophysiological analysis of kinetic effects of ligand 4. (A) Ligand 4 (50 μM) shifts the voltage dependence of channel opening to the right. Conductance measured from inward tails in 98 mM external K+. (n = 4, error bars indicate SEM). Data fit to a single Boltzmann as described in Methods. Control solution before exposure: max = 0.97 ± 0.04, Vmid = −42 ± 1 mV, m = 3.6 ± 0.9. During ligand 4 application: max = 0.68 ± 0.02, Vmid = −12 ± 1 mV, m = 11 ± 1.2. (B) Time course of reduction of current amplitude parallels slowing of channel opening after application of 50 μM ligand 4.