Scheme 1.
Equilibria involved in our protocol. (A) Indicator displacement assay (IDA). (B) Enantioselective indicator displacement assay (eIDA) using a chiral host for chiral diols with pyrocatechol violet (PV) as the indicator. H, host; I, indicator; G, guest/analyte; Δ Abs, absorbance change; [G]t, total guest concentration; ee, enantiomeric excess. The asterisk indicates the stereogenic center.