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Specific β-galactosidase activities of promoter fusions of the Fe–Fur system without Fe (in the presence of 200 μM the Fe chelator 2,2′-dipyridyl). In these conditions, the promoter of ryhB is fully active. Promoter activities of 4 target mRNAs are indicated by the gray bars, and their sum gives αm ≈ 20 + 19 + 12 + 2 = 53. The promoter activity of PryhB is αs ≈217(left black bar, ryhB+), therefore α = αs/αm ≈ 4. See SI Text and Table S1 for experimental details.
