Fig. 3.

DNA replication in Orc6 mutant. BrdU incorporation level (red) in third-instar larval brains (A) and in salivary glands of orc6³⁵ mutant (B) compared with wild-type or orc6³⁵/Cy heterozygous animals. (C) Immunofluorescence experiments using affinity-purified anti-Orc6 antibody on polytene chromosomes isolated from wild-type animals (red) and from orc6³⁵ mutant (green) show the presence of Orc6 at specific loci along the chromosome length and in the nucleolus (N). (Scale bars: A and B, 100 μm; C, 50 μm.)