Table 1.
Effect of Orc6 transgenes on G1 arrest of the orc635 neuroblasts
| Fly genotype | No. of cells |
G1:S/G2/M ratio | |
|---|---|---|---|
| G1,1 centrosome | S/G2/M,2 centrosomes | ||
| orc635/Cy | 154 | 119 | 1.3:1 |
| orc635/orc635 | 208 | 28 | 7.4:1 |
| orc635/Cy, GFP-orc6 | 286 | 250 | 1.1:1 |
| orc635/orc635, GFP-orc6 | 234 | 211 | 1.1:1 |
| orc635 /Cy, GFP-orc6-200 | 108 | 93 | 1.2:1 |
| orc635/orc635, GFP-orc6-200 | 324 | 33 | 9.8:1 |
| orc635/Cy, GFP-orc6-220 | 267 | 243 | 1.1:1 |
| orc635/orc635, GFP-orc6-220 | 235 | 187 | 1.2:1 |
| orc635/Cy, GFP-orc6-WK228AA | 176 | 144 | 1.2:1 |
| orc635/orc635,GFP-orc6-WK228AA | 256 | 236 | 1.1:1 |
| orc635/Cy, GFP-Hs-orc6 | 141 | 139 | 1.01:1 |
| orc635/orc635, GFP-Hs-orc6 | 317 | 221 | 1.4:1 |
Several independent fly stocks have been tested for each GFP-Orc6 transgene. In every case, tested stocks have shown similar G1 to S/G2/M ratios.