Fig. 3.
In vitro assay for identification of NDPS1 product using GC-MS. (A) Purified NDPS1 was incubated with IPP and DMAPP for 30 min at 30 °C, after which alkaline phosphatase was added to the reaction and the solution incubated overnight and then analyzed. (B) Authentic nerol standard. (C) Authentic geraniol standard. (D) Commercial GPP was treated with alkaline phosphatase as in A. GC-MS chromatograms show the detection of m/z = 93.
