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. Author manuscript; available in PMC: 2009 Jul 15.
Published in final edited form as: J Immunol. 2008 Jul 15;181(2):1429–1437. doi: 10.4049/jimmunol.181.2.1429

Figure 4. Listeria monocytogenes and Staphylococcus aureus N-formyl peptides are selective agonists for mFPR1.

Figure 4

A, RT-PCR detection of the transcripts of mouse formyl peptide receptors in neutrophils (PMN). The sizes of the individual transcripts, after PCR amplification, are indicated by the cDNA controls included in a parallel experiment. Data are representative of three separate experiments. B-D, Agonist-induced Ca2+ mobilization in RBL-mFPR1 cells (left panels) and RBL-mFPR2 cells (right panels) that were stimulated with WKYMVm (W-pep, B), fMIVIL (C), and fMIFL (D). All 3 peptides were used at 100 nM in assays using RBL-mFPR1. In assays using RBL-mFPR2, WKYMVm was used at 100 nM, and fMIVIL and fMIFL were each used at 1 μM. ATP, used in (B) to demonstrate equal loading of the fluorescent dye, was used at 1 μM. One representative set of tracings, from assays done on the same day, is presented out of a total of three separate experiments.