Table 1. Summary of crystal parameters, data-collection and refinement statistics for mitoNEET purified from an sfGFP fusion.

Values in parentheses are for the highest resolution shell.

Space group	P212121
Unit-cell parameters (Å, °)	a = 50.74, b = 48.48, c = 59.25, α = 90.00, β = 90.00, γ = 90.00
Data collection	λ1, native
Wavelength (Å)	1.1158
Resolution range (Å)	38.5–1.40
No. of observations	169484
No. of unique reflections	26874
Completeness (%)	91.1 (76.3)
Mean I/σ(I)	19.60 (4.3)
Rmerge on I† (%)	5.9 (32.7)
Model and refinement statistics
Data set used	λ1
Cutoff criterion	|F| > 0
Resolution range (Å)	38.5–1.40
No. of reflections (total)	26847‡
No. of reflections (test set)	1371
Completeness (%)	91.3
Rcryst§	0.202
Rfree¶	0.244
Stereochemical parameters
Restraints (r.m.s. observed)
Bond angle (°)	1.66
Bond length (Å)	0.012
Average isotropic B value (Å2)	5.64
ESU†† based on Rfree (Å)	0.078

^† R _merge = , where I _i(hkl) is the scaled intensity of the ith measurement and 〈I(hkl)〉 is the mean intensity for that reflection.

^‡Typically, the number of unique reflections used in refinement is slightly lower than the total number that were integrated and scaled. Reflections are excluded owing to systematic absences, negative intensities and rounding errors in the resolution limits and unit-cell parameters.

^§ R _cryst = , where F _calc and F _obs are the calculated and observed structure-factor amplitudes, respectively.

^¶ R _free is as for R _cryst, but calculated using 5% of the total reflections chosen at random that were omitted from refinement.

^††ESU, estimated overall coordinate error.