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. Author manuscript; available in PMC: 2009 Jul 3.

Published in final edited form as: J Hepatol. 2006 Oct 6;46(2):222–229. doi: 10.1016/j.jhep.2006.08.012

Tannic acid modulates the expression of membrane pumps involved in drug efflux. Panel A. Mz-ChA-1 cells were incubated with varying concentrations of tannic acid. After 48 hours, cells were lysed and expression of PGP, MRP-1 and MRP-2 assessed by western blots. The panel shows a representative immunoblot, whereas the figures represent quantitative data for expression relative to β-actin from 3 separate blots. Panel B. MzChA-1 cells were incubated with tannic acid at the indicated concentrations. After 48 hours, RNA was isolated, and cDNA obtained by reverse transcription. Real-time PCR was performed to quantitate the mRNA expression of PGP, MRP-1 and MRP-2. For each condition, mRNA expression was normalized to β-actin and expressed relative to that of diluent alone controls. A decrease in expression of these membrane pumps was noted (p<0.05 for all conditions relative to expression in untreated controls). Data represent the mean and 95% confidence intervals from four studies.

Tannic acid modulates the expression of membrane pumps involved in drug efflux. Panel A. Mz-ChA-1 cells were incubated with varying concentrations of tannic acid. After 48 hours, cells were lysed and expression of PGP, MRP-1 and MRP-2 assessed by western blots. The panel shows a representative immunoblot, whereas the figures represent quantitative data for expression relative to β-actin from 3 separate blots. Panel B. MzChA-1 cells were incubated with tannic acid at the indicated concentrations. After 48 hours, RNA was isolated, and cDNA obtained by reverse transcription. Real-time PCR was performed to quantitate the mRNA expression of PGP, MRP-1 and MRP-2. For each condition, mRNA expression was normalized to β-actin and expressed relative to that of diluent alone controls. A decrease in expression of these membrane pumps was noted (p<0.05 for all conditions relative to expression in untreated controls). Data represent the mean and 95% confidence intervals from four studies.