Figure 4.

High dosage of TEF5 reduces efficiency of UAA readthrough in the sup35 mutants. The strains 8V-H80-168 (sup35-168^ts) and 8V-H80-196 (sup35-196^ts) were transformed with one of the multicopy plasmids YEplac181-TEF5Δi (TEF5-Δi↑), YEplac181-SUP35C (SUP35C↑) or YEplac181 (empty vector) and then with the appropriate dual luciferase reporter plasmids. The transformants with plasmid pairs were grown and readthrough measured as described in legend to Figure 3 and in Methods. The difference in UAA readthrough in the mutant sup35-196^ts strain with the YEplac181-TEF5Δi and YEplac181 plasmids is statistically significant (P ≤ 0.1).