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. Author manuscript; available in PMC: 2010 Jun 11.
Published in final edited form as: Neuron. 2009 Jun 11;62(5):641–654. doi: 10.1016/j.neuron.2009.04.025

Figure 4. Transactivation, but not RARE-binding, by RA-bound RAR is required for Ngn2 to specify motor neurons.

Figure 4

(A) Schematic representation of RARα CoIP assays using IP with anti-HA antibody followed by immunoblotting with anti-Flag antibody in HEK293 cells transfected with Flag-Ngn2 and either HA-RARDBDmt or HA-RARDBDmtΔAF2. Both RARDBDmt and RARDBDmtΔAF2 associate with Ngn2 independently of RA. (C-Q) Immunohistochemical analysis of ectopic motor neuron induction or formation of endogenous motor neurons (ventral motor neurons below dotted lines in E, H, K, N, Q) in HH stage 25 chick embryos electroporated with the indicated constructs on the top. (R, S) Quantitative analysis of ectopic motor neuron (MN) generation in the dorsal spinal cord (R) or formation of endogenous motor neurons in the ventral spinal cord (S). RARΔAF2 and RARDBDmtΔAF2, but not RAR wild-type or RARDBDmt, block the specification of motor neuron cell-type. Asterisk, p<0.001 in the two-tailed t-test. The error bars represent the standard deviation.