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. 2008 Nov 26;19(8):1857–1869. doi: 10.1093/cercor/bhn213

Figure 4.

Figure 4.

p35-deficient interneurons demonstrate impaired motility and morphology. (A–D) Coronal sections of E13.5 forebrain labeled for CB. CB+ interneurons migrate in clearly defined streams from the GE toward cortex, in both p35+/+ (A) and p35−/− (B) mice. However, fewer interneurons are observed in each stream (PPL and IZ) in p35−/− mice (D) compared with controls (C). Delay in CB+ interneuron migration as indicated by a shorter distance (E; mean percentage ± SEM) and fewer cells evident in the Cx (at E13.5; F) and hippocampus (at E14.5; F) of p35−/− mice compared with controls. Total number of CB+ cells is presented as mean number ± SEM. (G, H) Results of chemomigratory assay using the Boyden chamber. The chemotactic response of MGE-derived cells (E13.5) from p35+/+ or p35−/− mice toward a control (Neurobasal medium) or 10% FBS (G) or following treatment of p35+/+ MGE cells with DMSO or 10 μM Roscovitine and exposure to 10% FBS (H). A significant impairment in chemotactic response to FBS is observed upon downregulation of p35/Cdk5 (mean number of migrated cells ± SEM). (I, J) Morphology of E13.5 CB+ cells in p35+/+ (I) and p35−/− (J) mice. (K) Branch measurements of E13.5 CB+ cells shown as mean number of branches per cell ± SEM. A significant increase in the number of branches is observed in p35−/− mice (n = 305 cells) compared with controls (n = 420 cells). (L–N) Forebrains from p35+/+ and p35−/− mice, injected and electroporated into the MGE with pCAG (control green vector; injected into p35+/+, L, and injected into p35−/−, M) or pCAG together with p35 plasmid (injected into p35−/−, N). Abnormal branching of migrating cells observed in the p35−/− mice (23 forebrain slices) is rescued by expression of p35 (34 forebrain slices) (O). The number of branches per cell calculated as a percentage of the pCAG-transfected control (p35+/+ mice; 23 forebrain slices). Cx, cortex; LGE, lateral ganglionic eminence; VZ, ventricular zone. Error bars represent SEM. **P < 0.01 and ***P < 0.005; Student's t-test. Scale bars, 250 μm (A–B); 100 μm (C–D); 25 μm (I, J, L–N).