Table 1.

Estimation of cortical layers from the depth of recording

Cortical layers	Subject	Depth of recording (μm)
		Upper edge	Lower edge	Thickness
Layer I	H1	−82 ± 109	25 ± 107	107 ± 21
	H3	−320 ± 478	−96 ± 503	194 ± 224
	H2	−642 ± 537	−404 ± 545	238 ± 10
Layers II and III	H1	25 ± 107	610 ± 144	585 ± 103
	H3	−96 ± 503	753 ± 502	841 ± 849
	H2	−404 ± 545	454 ± 554	858 ± 124
Layer IV	H1	610 ± 144	822 ± 159	212 ± 42
	H3	753 ± 502	1050 ± 489	259 ± 297
	H2	454 ± 554	729 ± 586	275 ± 45
Layer V	H1	822 ± 159	1072 ± 204	250 ± 46
	H3	1050 ± 489	1382 ± 497	280 ± 331
	H2	729 ± 586	1021 ± 573	292 ± 44
Layer VI	H1	1072 ± 204	1355 ± 222	284 ± 26
	H3	1382 ± 497	1693 ± 536	288 ± 311
	H2	1021 ± 573	1309 ± 591	288 ± 79

Note: The depth was measured from the site where the first extracellular activity was observed at each penetration site. Thus, depth = 0 does not necessarily correspond to the surface of the cortex or the border between layers I and II.