Table 4.
Progress of transporter cloning and functional characterization in the Giacomini Laboratory
| Transporter | MW | Functional clone | Yeast expression | Solubilized | Purified | |
|---|---|---|---|---|---|---|
| 1 | hMATE1 | 61919 | X | ~0.8 mg/l | No | No |
| 2 | rMATE1 | 61436 | X | ~0.5 mg/l | Yes | No |
| 3 | hENT1 | 50216 | X | ~2 mg/l | Yes | Yes |
| 4 | hENT2 | 50110 | X | ~1.2 mg/l | ~50% | Yes |
| 5 | hENT3 | 51712 | X | ~0.8 mg/l | ~60% | No |
| 6 | hCNT1 | 71580 | X | ~1.0 mg/l | Yes | Yes |
| 7 | hCNT2 | 71991 | X | POOR | Yes | Yes |
| 8 | hCNT3 | 76925 | X | ~0.5 mg/l | Yes | No |
| 9 | hOCTN1 | 62151 | X | ~1.0 mg/l | ~50% | No |
| 10 | hOCT1 | 61150 | X | ~1.0 mg/l | Yes | No |
| 11 | hOCT2 | 62560 | X | ~0.8 mg/l | Yes | No |
| 12 | hOCT3 | 61276 | X | ~0.5 mg/l | ~60% | No |
| 13 | hABCG2 | 72309 | X | 1.0 mg/l | No | No |
| 14 | hMATE2 | – | X | Not tested | Not tested | Not tested |
| 15 | hOAT1 | 61812 | X | Not tested | Not tested | Not tested |
| 16 | hOAT2 | 59807 | X | Not tested | Not tested | Not tested |
| 17 | hOAT3 | 59853 | X | Not tested | Not tested | Not tested |
| 18 | hOAT4 | 62166 | X | Not tested | Not tested | Not tested |
| 19 | hOCTN2 | 62747 | X | Not tested | Not tested | Not tested |
| 20 | CTR1 | – | X | Not tested | Not tested | Not tested |