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. 2009 Jul 16;4(7):e6255. doi: 10.1371/journal.pone.0006255

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Ling et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Mia1p-GFP localized to mitotic spindles and interphase microtubules in crm1-809 Uch2p-mCherry expressing cells at 36°C but only to the nucleus at 18°C. (B) Positions of predicted NLS and NES on Mia1p. (C) Mia1p-ΔC17-GFP is enriched in the nucleus in interphase cells. (D) GFP-NES but not MutNES is excluded from the nucleus. Shown are single maximum intensity reconstructions of live cells. Scale bars = 5 µm.