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. Author manuscript; available in PMC: 2009 Jul 6.
Published in final edited form as: Biomaterials. 2007 Jul 20;28(31):4535–4550. doi: 10.1016/j.biomaterials.2007.06.016

Figure 10.

Figure 10

Variation in long-term hFOB morphology on tissue-culture grade polystyrene (TCPS) assessed by cross-sectional TEM (Panels A: Scale bar = 5 μm; B: Scale bar = 10 μm) showing formation of multiple cell layers. Note that apoptotic bodies were clearly evident after 30 days of culture. Apoptotic cells (green) among normal cells (red, Sytox Orange) visualized using confocal microscopy confirms an increase in apoptosis with culture age (Panels C, D: Scale bar = 50 μm). Percent apoptotic bodies noted in lower right of Panels C,D were estimated by image analysis (see ref. [112] for experimental details).