Figure 3.

PGC-1α repressed the LDLR promoter activity regardless of cholesterol. HepG2 cells were transfected with LacZ expression vector (400 ng), expression vectors (400 ng) for PGC-1α or mock vector (pcDNA3.1/HisC), and pLR1563-luc, as indicated. The transfected cells were incubated for 24 h in media containing 10% FBS (FBS), 10% lipoprotein deficient serum (LPDS) or serum free media (SFM), and harvested for luciferase assay. Normalized luciferase expressions from triplicate samples were calculated relative to the LacZ expressions, and the results were expressed as n-fold activation over the value obtained with the reporter alone. Values are mean ± SD of three independent duplicate experiments. ^*: P < 0.05 vs. mock control.