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. 2009 Jun 26;41(6):406–416. doi: 10.3858/emm.2009.41.6.046

Figure 6.

Figure 6

Effect of N-SREBP-2 dominant positive or PPARγ activation on the PGC-1α-repressed LDLR promoter activity. (A) Overexpression of N-SREBP2 dominates the repressive effect of PGC-1α. HepG2 cells were transfected with LacZ expression vector, expression vectors for PGC-1α (400 ng) and SREBP-2 dominant positive (N-SREBP-2), and pLR1563-luc (800 ng), as indicated. (B) Activation of PPARγ did not alter the inhibition by PGC-1α. HepG2 cells were transfected with LacZ expression vector, expression vectors for PGC-1α (400 ng) and PPARγ, and pLR1563-luc (800 ng), as indicated. The cells were treated for 24 h with either vehicle only (DMSO) or a PPARγ ligand troglitazone (TZD, 10 µM) in serum free media and harvested for luciferase assay. Normalized luciferase expressions from triplicate samples were calculated relative to the LacZ expressions, and the results were expressed as % control over the value obtained with the reporter alone. Values are mean ± SD of three independent duplicate experiments.