Figure 7.

Inhibition of p38-MAPK had no effect on the PGC-1α-repressed LDLR mRNA expression. HepG2 cells were infected with AdLacZ alone (AdPGC -) or AdPGC-1 (AdPGC +) for 24 h at a multiplicity of infection of 50. The infected cells in serum free media were then incubated with p38-MAPK inhibitor SB202190 (20 µM) or vehicle DMSO for 24 h, and harvested for total RNA preparation. RT-PCR (A) and Northern blot (B) analyses of PGC-1α or LDLR in HepG2 cells. The PCR fragments or hybridized signals of PGC-1α, LDLR and β-actin are shown. Equivalent loading of RNA was verified by (A) the β-actin PCR product or (B) 28S and 18S rRNAs on the agarose gel stained with ethidium bromide.