Table 2.

Superoxide production was measured by EPR spectroscopy

Groups	Db/db	Db/db Apocynin	db/db	db/db Apocynin
6 weeks	100 ± 4	101 ± 3	114 ± 6#	93 ± 4*
12 weeks	100 ± 3	102 ± 5	134 ± 7#	109 ± 6*
	Db/db	Db/db Apocynin rotenone	db/db	db/db Apocynin rotenone
18 weeks	116 ± 8#	97 ± 5&	151 ± 12#	110 ± 9*
	Db/db	Db/db Rotenone	db/db	db/db Rotenone
24 weeks	127 ± 11#	109 ± 6&	158 ± 17#	116 ± 8*

O₂^.− production was higher in isolated coronary arteries from db/db mice Vs. Db/db mice aged 6, 12, 18 and 24 weeks. Administration of NAD(P)H oxidase inhibitor apocynin reduced the production of O₂^.− in db/db mice at 6 and 12 weeks (n = 9); administration of apocynin and mitochondria respiratory chain inhibitor rotenone reduced the production of O₂^.− in Db/db and db/db mice at 18 weeks (n = 9); and administration of rotenone reduced the production of O₂^.− in Db/db and db/db mice at 24 weeks (n = 9)

Values are mean ± SE.

^#P < 0.05 Vs. Db/db mice;

^&P < 0.05 Vs. Db/db mice (18 and 24 weeks); and

^*P < 0.05 Vs. db/db