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. Author manuscript; available in PMC: 2009 Jul 6.
Published in final edited form as: Mutat Res. 2007 Nov 29;650(2):181–195. doi: 10.1016/j.mrgentox.2007.11.006

Table I.

Participating Laboratories.

Site Lab Code Chemicals Cytotoxicity Measurements Flow Cytometer Microscopy
Litron Laboratories, Rochester, NY L1 SUC, DEX, STS, MMC, VB, and ETOPO RS: Flow-NBR
RS: Fluorescein diacetate
RS: Coulter Counter
Population Doubling
%EMA+ Events		 BD
FacsCalibur running
CellQuest v3.3		 Olympus BH2, 10 × 40 Mag., Acridine orange staining
Novartis Pharma AG, Basel, Switzerland L2 SUC, MMC RS: Flow-NBR
RS: Trypan blue, Schilling camera
RS: Sysmex Counter
Population Doubling
%EMA+ Events		 BD
FacsCalibur running
CellQuest Pro v4.0.2		 Zeiss, 10 × 100x Mag., Feulgen + Congo Red staining
Johnson & Johnson, Pharmaceutical R&D, Beerse, Belgium L3 STS, ETOPO RS: Flow-NBR
RS: Coulter Counter
RS: NucleoCounter
Population Doubling
%EMA+ Events		 BD
FacsCalibur running
CellQuest Pro v5.2		 Zeiss Axioskop, 1.25 × 63 Mag., Giemsa staining
GlaxoSmithKline R&D, Hertfordshire, UK L4 DEX, VB RS: Flow-NBR
RS: Coulter Counter
Population Doubling
%EMA+ Events
Annexin Viability		 BD
FacsCalibur running
CellQuest Pro v5.2.1		 Leica DMR XA, 1.25 × 40 Mag., Acridine orange staining

Abbreviations: SUC = sucrose; DEX = dexamethasone; STS = staurosporine; MMC = mitomycin C; VB = vinblastine sulfate; ETOPO = etoposide; RS = relative survival; EMA = ethidium monoazide bromide; Flow-NBR = flow cytometric nuclei to bead ratio