Table II.
| Table II. A. Micronucleus Data: Mitomycin C. | ||||||||
|---|---|---|---|---|---|---|---|---|
| %MN: Microscopy | %MN: Flow |
|||||||
| Chemical | Lab | Conc. | Values | Mean | Fold Increase | Values | Mean | Fold Increase |
| Mitomycin C | L1 | 0 | 0.4, 0.3 | 0.35 | - | 0.15, 0.16 | 0.16 | - |
| 0.039 | 3.5, 2.9 | 3.20 | 9.1 | 1.86, 1.46 | 1.66 | 10.4 | ||
| 0.078 | 6.1, 5.3 | 5.70 | 16.3 | 4.54, 3.72 | 4.13 | 25.8 | ||
| 0.156 | 13.2, 8.5 | 10.85 | 31.0 | 7.47, 6.76 | 6.87 | 42.9 | ||
| 0.3125 | 19.7, 20.7 | 20.20 | 57.7 | 11.75, 17.05 | 14.40 | 90.0 | ||
| L2 | 0 | 0.5, 0.2 | 0.35 | - | 0.12, 0.09 | 0.11 | - | |
| 0.039 | 4.6, 3.8 | 4.20 | 12.0 | 4.01, 4.00 | 4.00 | 36.4 | ||
| 0.078 | 4.7, 6.0 | 5.35 | 15.3 | 10.79, 8.40 | 9.60 | 87.3 | ||
| 0.156 | 8.0, 7.6 | 7.80 | 22.3 | 15.16, 15.48 | 15.32 | 139.3 | ||
| 0.3125 | 8.2, 8.5 | 8.35 | 23.9 | 15.99, 17.43 | 16.71 | 151.9 | ||
| Table II. B. Micronucleus Data: Sucrose. | ||||||||
|---|---|---|---|---|---|---|---|---|
| %MN: Microscopy | %MN: Flow |
|||||||
| Chemical | Lab | Conc. | Values | Mean | Fold Increase | Values | Mean | Fold Increase |
| Sucrose | L1 | 0 | 0.2, 0.1 | 0.15 | - | 0.18, 0.20 | 0.19 | - |
| 625 | 0.3, 0.3 | 0.30 | 2.0 | 0.14, 0.22 | 0.18 | 0.9 | ||
| 1250 | 0.1, 0.2 | 0.15 | 1.0 | 0.20, 0.11 | 0.16 | 0.8 | ||
| 2500 | 0.5, 0.3 | 0.40 | 2.7 | 0.19, 0.17 | 0.18 | 0.9 | ||
| 5000 | 0.4, 0.1 | 0.25 | 1.7 | 0.20, 0.30 | 0.25 | 1.3 | ||
| L2 | 0 | 0.2, 0.1 | 0.15 | - | 0.22, 0.27 | 0.25 | - | |
| 625 | 0.2, 0.2 | 0.20 | 1.3 | 0.31, 0.13 | 0.22 | 0.9 | ||
| 1250 | 0.3, 0.3 | 0.30 | 2.0 | 0.34, 0.11 | 0.23 | 0.9 | ||
| 2500 | 0.1, 0.3 | 0.20 | 1.3 | 0.51, 0.36 | 0.44 | 1.8 | ||
| 5000 | 0.2, 0.3 | 0.25 | 1.7 | 0.31, 0.18 | 0.25 | 1.0 | ||
| Table II. C. Micronucleus Data: Etoposide. | ||||||||
|---|---|---|---|---|---|---|---|---|
| %MN: Microscopy | %MN: Flow |
|||||||
| Chemical | Lab | Conc. | Values | Mean | Fold Increase | Values | Mean | Fold Increase |
| Etoposide | L1 | 0 | 0.4, 0.4 | 0.40 | - | 0.15, 0.21 | 0.18 | - |
| 0.005 | 0.6, 0.7 | 0.65 | 1.6 | 0.23, 0.10 | 0.17 | 0.9 | ||
| 0.01 | 2.4, 2.4 | 2.40 | 6.0 | 0.50, 0.49 | 0.50 | 2.8 | ||
| 0.02 | 5.2, 5.1 | 5.15 | 12.9 | 1.29, 1.43 | 1.36 | 7.6 | ||
| 0.04 | 15.8, 14.3 | 15.05 | 37.6 | 5.19, 5.28 | 5.24 | 29.1 | ||
| 0.08 | ND | ND | ND | 13.07, 11.49 | 12.28 | 68.2 | ||
| L3 | 0 | 0.2, 0.1 | 0.15 | - | 0.16, 0.14 | 0.15 | - | |
| 0.005 | 2.1, 2.1 | 2.10 | 14.0 | 1.08, 0.66 | 0.87 | 5.8 | ||
| 0.01 | 3.9, 4.5 | 4.20 | 28.0 | 2.04, 1.81 | 1.93 | 12.9 | ||
| 0.02 | 10.7, 12.6 | 11.65 | 77.7 | 4.76, 4.22 | 4.49 | 29.9 | ||
| 0.04 | 22.2, 22.4 | 22.30 | 148.7 | 11.16, 10.28 | 10.72 | 71.5 | ||
| 0.08 | 32.4, 31.1 | 31.75 | 211.7 | 16.81, 17.65 | 17.23 | 114.9 | ||
| Table II. D. Micronucleus Data: Staurosporine. | ||||||||
|---|---|---|---|---|---|---|---|---|
| %MN: Microscopy | %MN: Flow |
|||||||
| Chemical | Lab | Conc. | Values | Mean | Fold Increase | Values | Mean | Fold Increase |
| Staurosporine | L1 | 0 | 0.2, 0.1 | 0.15 | - | 0.18, 0.24 | 0.21 | - |
| 0.0016 | 0.1, 0.3 | 0.20 | 1.3 | 0.12, 0.12 | 0.12 | 0.6 | ||
| 0.0031 | 0.5, 0.4 | 0.35 | 2.3 | 0.07, 0.16 | 0.12 | 0.6 | ||
| 0.0062 | 0.3, 0.5 | 0.40 | 2.7 | 0.10, 0.17 | 0.14 | 0.7 | ||
| 0.0125 | 0.3, 0.3 | 0.30 | 2.0 | 0.30, 0.28 | 0.29 | 1.4 | ||
| 0.0250 | ND | ND | ND | 0.93, 1.53 | 1.23 | 5.9 | ||
| L3 | 0 | 0.2, 0.3 | 0.25 | - | 0.17, 0.14 | 0.16 | - | |
| 0.0016 | 0.2, 0.3 | 0.25 | 1.0 | 0.14, 0.08 | 0.11 | 0.7 | ||
| 0.0031 | 0.6, 0.4 | 0.50 | 2.0 | 0.20, 0.17 | 0.19 | 1.2 | ||
| 0.0062 | 1.1, 1.0 | 1.05 | 4.2 | 0.23, 0.23 | 0.23 | 1.4 | ||
| 0.0125 | 1.1, 0.8 | 0.95 | 3.8 | 0.27, 0.37 | 0.32 | 2.0 | ||
| 0.0250 | 1.1, 0.7 | 0.90 | 3.6 | 0.74, 1.07 | 0.91 | 5.7 | ||
| Table II. E. Micronucleus Data: Vinblastine. | ||||||||
|---|---|---|---|---|---|---|---|---|
| %MN: Microscopy | %MN: Flow |
|||||||
| Chemical | Lab | Conc. | Values | Mean | Fold Increase | Values | Mean | Fold Increase |
| Vinblastine | L1 | 0 | 0.1, 0.1 | 0.10 | - | 0.26, 0.21 | 0.24 | - |
| 0.9 | 0.1, 0.3 | 0.20 | 2.0 | 0.36, 0.76 | 0.56 | 2.3 | ||
| 1.0 | 0.5, 0.4 | 0.45 | 4.5 | 0.58, 0.71 | 0.65 | 2.7 | ||
| 1.1 | 0.7, 1.0 | 0.85 | 8.5 | 1.03, 1.98 | 1.51 | 6.3 | ||
| 1.2 | 0.8, 1.3 | 1.05 | 10.5 | 1.16, 1.82 | 1.49 | 6.2 | ||
| 1.3 | 1.2, 1.4 | 1.30 | 13.0 | 6.31, 2.36 | 4.34 | 18.1 | ||
| L4 | 0 | 0.2, 0.3 | 0.25 | - | 0.16, 0.10 | 0.13 | - | |
| 0.9 | 0.4, 0.3 | 0.35 | 1.4 | 0.19, 0.23 | 0.21 | 1.6 | ||
| 1.0 | 0.6, 0.5 | 0.55 | 2.2 | 0.35, 0.33 | 0.34 | 2.6 | ||
| 1.1 | 0.6, 0.6 | 0.60 | 2.4 | 0.51, 0.51 | 0.51 | 3.9 | ||
| 1.2 | 0.7, 1.2 | 0.95 | 3.8 | 0.90, 1.34 | 1.12 | 8.6 | ||
| 1.3 | 0.7, 1.7 | 1.20 | 4.8 | 1.96, 1.54 | 1.75 | 13.5 | ||
| Table II. F. Micronucleus Data: Dexamethasone. | ||||||||
|---|---|---|---|---|---|---|---|---|
| %MN: Microscopy | %MN: Flow |
|||||||
| Chemical | Lab | Conc. | Values | Mean | Fold Increase | Values | Mean | Fold Increase |
| Dexamethasone | L1 | 0 | 0.2, 0.1 | 0.15 | - | 0.28, 0.29 | 0.28 | - |
| 7.8 | 0.3, 0.4 | 0.35 | 2.3 | 0.20, 0.36 | 0.28 | 1.0 | ||
| 15.625 | 0.2, 0.3 | 0.25 | 1.7 | 0.29, 0.35 | 0.32 | 1.1 | ||
| 31.25 | 0.3, 0.5 | 0.40 | 2.7 | 0.58, 0.43 | 0.50 | 1.8 | ||
| 62.5 | 0.3, 0.6 | 0.50 | 3.3 | 3.32, 1.84 | 2.08 | 7.3 | ||
| L4 | 0 | 0.2, 0.4 | 0.30 | - | 0.62, 0.34 | 0.48 | - | |
| 15.625 | 0.4, 0.5 | 0.45 | 1.5 | 0.17, 0.65 | 0.41 | 0.9 | ||
| 31.25 | 0.3, 0.5 | 0.40 | 1.3 | 0.31, 0.23 | 0.27 | 0.6 | ||
| 62.5 | 0.3, 0.2 | 0.25 | 0.8 | 0.56, 0.45 | 0.50 | 1.0 | ||
| 125 | 0.4, 0.6 | 0.50 | 1.7 | 1.86, 2.15 | 2.01 | 4.2 | ||
Note: shading indicates concentration is excessively cytotoxic based on Flow-NBR assessment.
ND = not determined; too cytotoxic.