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(a–d) Immuno-EM of gradient-purified VLPs. Localization of p40 protein on L. heterotoma VLPs (a, b) and p47.5 on L. victoriae VLPs (c). For the negative control (d), primary antibody was omitted. (e) Binding of primary antibody as measured by counting the number of gold beads adhering to the surface (S) or internal (I) regions of 22 VLPs from either wasp species. The sum of these two numbers is represented as the total (T) binding of gold beads to the VLPs.
