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Overexpression of EphA2 increases MVD. EphA2 expression in tumor tissue collected from xenografts of A2780, A2780-neo and two clones of A2780-EphA2 cells were determined by IHC (A) and Western blot (B). Tumors were subjected to IHC for CD31(C) to allow identification of endothelial cells. A lumen with positive CD31 staining was counted as a single microvessel.
