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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Mol Pharm. 2009;6(3):971–977. doi: 10.1021/mp900006h

Figure 1.

Figure 1

Preparation of cationic liposome (CL) loaded with D-(KLAKLAK)2 peptide and G3139 ODN. (a) Agarose gel electrophoresis confirmed the complex formation between peptide and ODN and subsequent entrapment of the complex with cationic liposome. Lane1: naked peptide/ODN complexes, nontreated; Lane2: naked peptide/ODN complexes, treated with heparin; Lane 3: CL loaded with peptide/ODN complexes, nontreated; Lane 4: CL loaded with peptide/ODN complexes, treated with heparin and Triton X-100; (b) Size exclusion chromatography (SEC) using FAM-D-(KLAKLAK)2 tracer revealed that about 54 % of peptide was loaded in CL. Free peptide was separated from CL-loaded peptide on a Sepharose CL4B column. Entrapped D-(KLAKLAK)2 eluted with CL at void volume. Without pre-complexation, the loading of peptide into CL was insignificant.