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. Author manuscript; available in PMC: 2009 Jul 6.
Published in final edited form as: Mol Biochem Parasitol. 2007 Sep 15;156(2):246–254. doi: 10.1016/j.molbiopara.2007.09.001

Fig. 2.

Fig. 2

RNAi-induced downregulation of TbCE1 and TbCGM1. (A) Two independent clonal cell lines expressing TbCE1 dsRNA were grown in the absence (lanes 1 and 3) or presence (lanes 2 and 4) for 24 hr and total cell extracts were assayed for enzyme-GMP complex formation. (B) Same assay as in panel (A), except that TbCGM1 was downregulated (lanes 2 and 4). (C) Cell densities were measured 24, 48, 72, 90 and 96 hr after induction of RNAi with tetracycline in a clonal cell line expressing double-stranded RNA for either TbCE1 or TbCGM1. Since the growth curves of uninduced cells were essentially identical to wild-type cells (wt), they are represented as one.

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