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. Author manuscript; available in PMC: 2009 Jul 6.
Published in final edited form as: Mol Biochem Parasitol. 2007 Sep 15;156(2):246–254. doi: 10.1016/j.molbiopara.2007.09.001

Fig. 3.

Fig. 3

TbCE1 downregulation by RNAi. (A) Total RNA isolated from uninduced (lanes 1–5) or TbCE1-RNAi cells induced for 8 days (lanes 6–10) was immunoprecipitated with anti-m7G and anti-TMG antibodies and the input (I), supernatant (S) and pellet (P) fractions were assayed by primer extension of the U2 snRNA (U2) and U3 snoRNA (U3). (B) The TbCE1 RNAi cell line was induced with tet for the indicated number of days (lanes 2–4) and total RNA was assayed by primer extension with an SL intron-specific primer. The position of fully-modified (fm SL) and hypomodified (hm SL) SL RNA is indicated. RNA isolated from sinefungin-treated cells served as a control for hypomodified SL RNA (lane 1). A U6 snRNA-specific primer was included in the reactions to control for RNA amounts and quality.

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