Fig. 6.
TbCgm1 is recruited to the SL RNA genes in vivo. Chromatin immunoprecipitations were performed with antibodies directed against the BB2 epitope tag present on TbCgm1 (lanes 3 and 6) or TbCe1 (lanes 9 and 12), or with no antibodies as a control (lanes 2, 5, 8 and 11). PCR amplification of the immunoprecipitates was done with primers upstream of the SL promoter (nt −608 to −530) or within the promoter (nt −56 to +82). Numbering is with respect to the SL RNA transcription start site. An aliquot of the total input is also shown (lanes 1, 4, 7 and 10).