Skip to main content
. Author manuscript; available in PMC: 2009 Jul 6.
Published in final edited form as: Eur J Immunol. 2008 Feb;38(2):518–527. doi: 10.1002/eji.200737430

Figure 5.

Figure 5

Total thymocytes were stimulated by crosslinking of CD3 with 10 µg/mL anti-CD3 Ab and anti-hamster Ig Ab for 5 or 20 min (upper panels) or by incubation with 25 ng/mL PMA for 20 min (lower right panel). Cells were treated for 30 min with 10 µM U0126 prior to TCR stimulation (lower left panel). Histograms show profiles of phospho-ERK in B-Raf+/+ (filled gray) and B-Raf−/− (bold line) cells gated on CD4+CD8+ DP cells from DN cell-chimeric mice. Dotted lines indicate the status of phospho-ERK in unstimulated B-Raf+/+ DP cells. The data are from one representative of three independent experiments.