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. Author manuscript; available in PMC: 2009 Jul 6.
Published in final edited form as: J Control Release. 2007 May 13;122(3):385–391. doi: 10.1016/j.jconrel.2007.05.005

Figure 4. ABCG2 and ABCB1 activity in freshly isolated therapy naïve non-small cell lung cancer.

Figure 4

Antibody stained suspended tumor cells were incubated simultaneously with Hoechst 33342 (8 microM) plus R123 (0.13 microM) for 90 minutes at 37°C. Propidium iodide (PI, 10 microg/mL) was added immediately before sample acquisition. All events were gated on PI excluding (live), non-hematopoietic CD45−) singlets (doublet discrimination based on forward light scatter pulse analysis). A total of 2.3 million cells were acquired.