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. 2008 Aug;58(4):324–340.

Table 2.

Examples of immunologic reagents and assays specifically designed for the guinea pig model

Immune mediator Reagent or assay Reference
B cells Flow cytometry 276
Basophils Flow cytometry 277
CCL5 (RANTES) Recombinant form 266
Recombinant form 47
CD4+ T cells Flow cytometry 276
CD8+ T-cells Flow cytometry 276
CD4CD8 T cells Flow cytometry 276
Eosinophils Flow cytometry 277
GM-CSF Reverse Transcription-PCR 319
Granulocytes Flow cytometry 276
IFNγ Recombinant form 136
Reverse Transcription-PCR 319
Real Time-PCR 53
Bioassay 321
IgG ELISA 143
Monoclonal antibody 174
Inducible nitric oxide synthase Reverse Transcription-PCR 319
IL1β Reverse Transcription-PCR 319
IL2 ELISA 1
Cloned and sequenced 251
Reverse Transcription-PCR 319
Northern Blot Analysis 137
IL8 Real Time-PCR 167
Recombinant form 168
Cloned 48
IL10 Cloned and sequenced 251
Reverse Transcription-PCR 319
IL12 Cloned and characterized 262
IL12p40 Cloned and sequenced 251
Reverse Transcription-PCR 319
Real Time-PCR 53
Kurloff cells Flow cytometry 276
Macrophages Antibody PM1K 122
Antibody MR1 154
Monoclonal antibodies 342, 322, and 249 171
MHC class II+ activated T cells Flow cytometry 276
Monocytes Flow cytometry 276
Neutrophils Flow cytometry 277
T cells Flow cytometry 276
TGFβ Cloned and sequenced 251
Reverse Transcription-PCR 319
TNFα Recombinant form, antibody 54
Recombinant form 160
Reverse Transcription-PCR 319