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. 2009 Jul 14;4(7):e6232. doi: 10.1371/journal.pone.0006232

Figure 2. Southern blot analyses of C. perfringens random mutants obtained after electroporation with EZ-Tn5 transposomes.

Figure 2

After selection on BHI plates containing Erm (40 µg/ml), DNA was extracted from strain 13 transformants. Following digestion with EcoRI (A) or XbaI (B), the digested DNA was electrophoresed and blotted to a nylon membrane. DNA on the membranes was then hybridized with a Dig-labeled erm probe, as found in the C. perfringens-modified EZ-Tn5, and blots were developed as described in the Materials and Methods. Size of DNA fragments, in kilobases (kb), is shown at left.