Figure 5. Early transcription of pfoA and plc genes is regulated by the C. perfringens agr locus.

Total RNA was extracted from a 2 h TGY culture of the wt strain 13 (S13), CPJV501 (ΔagrB), CPJVp1, CPJVp2 or CPJVp3. Quantitative RT-PCR was then performed with 20 ng of each RNA and primers that amplified the (A) pfoA gen (pfoAF1 and pfoAR1) or the (B) plc gene (cpaF and cpaR). Average C_T values were normalized to the polC gene and the fold differences were calculated using the comparative C_T method (2^−ΔΔC _T) [44]. Values below each bar indicate the calculated fold change relative to the wt strain 13. Panels shown are representative of three independent experiments.