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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Comp Biochem Physiol C Toxicol Pharmacol. 2008 Sep 5;149(3):307–316. doi: 10.1016/j.cbpc.2008.08.007

Figure 2.

Figure 2

SDS-PAGE on 10–20% Tricine gels. A) C. o. helleri crude venoms. A total of 250 μg of venom were run at 125V for 90 min. Lane 1: Riverside 1; Lane 2: Riverside 2; Lane 3: San Bernardino 1; Lane 4: San Bernardino 2; Lane 5: San Bernardino 3; Lane 6: SeeBlue Plus2 (Invitrogen™). B) Cationic exchange fraction FV. A total of 5 μg of fraction FV collected with and without EDTA, under reduced and non-reduced conditions were run at 125V for 90 min. Lane 1: FV collected without 10 mM EDTA and run under reducing conditions; Lane 2: FV collected in 10 mM EDTA and run under reducing conditions; Lane 3: FV collected without EDTA and run under non-reducing conditions; Lane 4: FV collected in EDTA and run under non-reducing conditions; Lane 5: SeeBlue Plus2 Markers (Invitrogen™). Gels were stained with RapidStain.