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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Comp Biochem Physiol C Toxicol Pharmacol. 2008 Sep 5;149(3):307–316. doi: 10.1016/j.cbpc.2008.08.007

Figure 4.

Figure 4

A) Fibrinolytic (F1, 9, 16), kallikrein-like (F2, 6–16), and thrombin-like (F4 to F8, F12 and F13) activities presented in anion exchange chromatography fractions obtained from San Bernardino 3 venom. B) Cationic exchange chromatography of fraction 1 collected from crude C. o. helleri venom (Fig. 4A). A total of 125 μL (4.5 mg/mL) of fraction 1 was injected into a Waters Protein Pak™ SP 5PW (7.5 × 75 mm) column. The equilibrium buffer was 0.02M Tris-HCl, pH 8.0. The elution was performed with a linear gradient of 0 to 0.5 M NaCl over 60 min with a flow rate of 1 mL/min. Proteins were detected at 280 nm. Black shaded areas indicate active fractions.