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. Author manuscript; available in PMC: 2010 May 15.
Published in final edited form as: Cell. 2009 May 15;137(4):623–634. doi: 10.1016/j.cell.2009.02.037

Figure 4. KRAS/RAF1 Regulation of β-catenin requires the Activity of RAC1.

Figure 4

(A, B) SW-480 cells transfected with vehicle or myc-tagged (A, S191A or S605A) or flag-tagged (B, S552A or S675A) β-catenin mutants were stained for DNA (magenta) and α-myc (A, green) or α-flag (B, green). (C) SW-480 cells were transfected with RAC1-directed siRNA, DN-RAC1, DN-Cdc42 or treated with the RAC1-specific inhibitor NSC23766 were stained for DNA (magenta) and β-catenin (green). (D) SW-480 cells were subjected to an MTT assay (*p<0.05 vs DMSO). (E) SW-480 cells were transfected with vehicle, KRASG12V-targeted siRNA, RAF1 siRNA or DN-MEK1 and subjected to a RAC1 activity assay. The western blot was probed for RAC1 (top). Control lysates were probed for total RAC1 (bottom). (F) SW-480 cells treated as above were stained for phospho-cJun. (G) Human 293 cells transfected with constitutively active RAC1 and treated with PGE2 or DMSO were stained for DNA (magenta) and β-catenin (green). All images were captured using the same exposure and are representative of at least three independent experiments. (Scale Bar: 10μm)