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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: J Autoimmun. 2009 Jan 21;32(1):70–77. doi: 10.1016/j.jaut.2008.12.001

Figure 5. No reduction of memory CD8 T cells and their response in the absence of CD103.

Figure 5

CD103+/+, CD103+/−, and CD103−/− mice were infected with 104 PFU LCMV and spleens were collected and analyzed 45 days after infection. A-B: Cells were stained for the presence of GP33 and NP396 pentamers and analyzed by flow cytometry. The total number of GP33 and NP396 cells was calculated after multiplication of their frequency with the total spleen number (A). The GP33 or NP396 cell frequency (B) was evaluated after gating in the CD8+CD19− cell population. C-D: Spleen cells were cultured with the LCMV MHC-I peptides GP33 (not shown) and NP396 before staining for intracellular IFNγ of TNF. The total number of antigen-specific cells per spleen was calculated by multiplying the percent CD8/IFNγ double positive cells by the total number of cells isolated from the spleen of each mouse. Representative data are from one out of two experiments. Differences are not statistically significant.