Table 3.
Antibodies for Western blot and immunohistochemistry
| Gene | Application | Protein forms detected by WB | Species | Type/clone | Dilution In WB | Dilution in IHC-P and ICC | Company |
|---|---|---|---|---|---|---|---|
| MMP-1 | WB, IHC | latent and active | rabbit | polyclonal | 1:750 | 1:100 | Biozol |
| MMP-2 | WB, IHC | latent and active | rabbit | polyclonal | 1: 1000 | 1:100 | Abcam |
| MMP-3 | WB, IHC, ICC | latent and active | mouse | SPM 293 | 1: 500 | 1:50 | Abcam |
| MMP-7 | WB | latent and active | mouse | 111433 | 1: 500 | - | Abcam |
| MMP-8 | WB | latent and active | mouse | 115-13D2 | 1: 1000 | - | Chemicon |
| MMP-9 | WB | latent and active | mouse | 9D4.2 | 1: 500 | - | Chemicon |
| MMP-10 | WB, IHC, ICC | latent and active | mouse | IVC5 | 1: 500 | 1:100 | Chemicon |
| MMP-11 | WB | latent and active | mouse | SL 3.01 | 1: 500 | - | Abcam |
| MMP-11 | IHC, ICC | latent and active | mouse | SPM 199 | - | Prediluted | Biozol |
| MMP-12 | WB | latent and active | rabbit | polyclonal | 1: 1000 | - | Abcam |
| MMP-13 | WB, IHC | latent and active | mouse | 87512 | 1: 500 | 1:100 | R&D |
| MMP-14 | WB, IHC, ICC | latent and active | rabbit | polyclonal | 1: 500 | 1:75 | Abcam |
| MMP-15 | WB, IHC, ICC | latent and active | rabbit | polyclonal | 1: 500 | 1:100 | Abcam |
| MMP-19 | WB | latent and active | rabbit | polyclonal | 1: 3000 | - | Biozol |
| MMP-19 | IHC, ICC | latent and active | rabbit | polyclonal | - | Prediluted | Biozol |
| MMP-23 | WB | latent and active | rabbit | polyclonal | 1: 1000 | - | Abcam |
| MMP-24 | WB | latent and active | rabbit | polyclonal | 1: 1000 | - | Abcam |
| MMP-27 | WB | not specified | rabbit | polyclonal | 1: 1000 | - | Abcam |
| MMP-28 | WB | not specified | rabbit | polyclonal | 1: 1000 | - | Abcam |
| β-actin | WB | b-actin | mouse | M/Abcam 8226 | 1: 10.000 | - | Abcam |
WB: Western blot; IHC-P: Immunohistochemistry (formalin-fixed paraffin-embedded section),
ICC: Immunocytochemistry