Fig. 5.

Induction of BBB permeability after i.c. infection with SPBAANGAS-GAS-GAS. 129/SvEv mice were injected with 10⁷ FFU of SPBAANGAS-GAS-GAS into the right hemisphere. BBB permeability to the fluid phase marker Na-fluorescein was assessed 6 and 8 days later in the right and left cortex and in cerebellum. Levels of mRNAs specific for the T cell marker CD4 and the B cell marker CD19 in the same tissues were assessed by quantitative RT-PCR. BBB permeability is expressed as the amount of Na-fluorescein detected in infected CNS tissues normalized to the amount in uninfected CNS tissue (A). CD4 (B) and CD19 (C) mRNA levels are expressed as the fold increase in infected over the levels detected in uninfected brain tissue. Significance of differences between the signals in normal and infected tissues were assessed by the Mann–Whitney test. *, P < 0.05; **, P < 0.01.