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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Bone. 2009 May 3;45(2):330–338. doi: 10.1016/j.bone.2009.04.245

Figure 2. Mustn1 and chondrogenic marker gene expression is differentially regulated during RCJ cell differentiation.

Figure 2

Expression of Mustn1 (A), and Sox9, a pre-chondrocyte cell marker, Collagen II, a proliferating chondrocyte cell marker, and Collagen X, a hypertrophic chondrocyte cell marker (B) as assayed via qPCR during RCJ differentiation. Confluent RCJ cells were stimulated to differentiate at Day 0 and mRNA was isolated and assayed at the days indicated. All bars represent average values of raw gene expression normalized to β2-microglobulin in pooled mRNA (n=3). Error bars indicate standard deviation (st. dev.) between qPCR runs (n=3). C. Photomicrographs of tissue culture dishes showing respective Alcian stained cell lines to indicate degree of differentiation on Day 0, 5, 10, and 14.