Table 1.
List of primers and conditions used for qPCR.
| Target Gene | Accession Number | Primer Sequence | Amplicon Size | Tm(oC) |
|---|---|---|---|---|
| β-2microglobulin | NM 012512 | 5′ - TGGTGTGCTCATTGCTATTC 3′ - CTCTGAAGGAGCCCAAAAC |
152 | 58 |
| Mustn1 | NM 181390 | 5′ - GCTTTTCCTCTGCCACCTC 3′ - ATTCCCCGACCCACCTC |
129 | 58 |
| Collagen II | NM 012929 | 5′ - TGTGCTTCTTCTCCTTGCTC 3′ - GACCTGAAACTCTGCCACC |
187 | 58 |
| Collagen X | AJ131848 | 5′ - ACCTGGGGCAACTTAGAAAA 3′-CAGTGGAATAGAAGGCACACA |
179 | 58 |
| Sox9 | AB073720 | 5′ - CCGACACGGAGAACACAC 3′ – CAGTCATAGCCCTTCAGCAC |
98 | 58 |